Skip to content
PubMed This is a summary of 12 peer-reviewed journal articles Updated
Endocrinology

Diagnosis and Labs: Putting the Pieces Together

At a Glance

Sheehan syndrome is diagnosed by confirming a history of severe childbirth bleeding, identifying symptoms like a lack of breast milk, and testing for low pituitary hormones. Diagnosis usually requires a morning hormone panel and a pituitary MRI to check for a shrunken gland known as an empty sella.

Diagnosing Sheehan syndrome is often like putting together a puzzle. Because the damage to the pituitary gland can be partial or complete, your lab reports may show a wide range of results. Doctors use a combination of your medical history, specialized blood tests, and brain imaging to confirm the diagnosis [1][2].

(Back to Home)

The Diagnostic Criteria

The primary “blueprint” for a Sheehan syndrome diagnosis includes:

  1. A History of Severe Bleeding: A documented event of heavy blood loss (postpartum hemorrhage) during childbirth [1].
  2. Clinical Clues: Signs like agalactia (inability to produce breast milk) and amenorrhea (failure of periods to return) [3].
  3. Lab Confirmation: Blood tests showing that the pituitary gland is not producing enough hormones [4].

Your Hormone Panel Checklist

To get a full picture of your pituitary health, your doctor will likely order a “morning panel” (usually around 8:00 AM, when hormone levels are peak). You should check your lab reports for the following [4][5]:

  • Adrenal Function: Cortisol and ACTH (the hormone that tells your adrenals to work).
  • Thyroid Function: TSH and Free T4 (looking for “secondary” hypothyroidism).
  • Reproductive Function: FSH, LH, and Estrogen.
  • Milk Production: Prolactin (often very low or undetectable in Sheehan syndrome).
  • Growth: Growth Hormone (GH) or IGF-1.
  • Water Balance: Sodium and Osmolality (to check if the back part of the pituitary is involved) [6].

Understanding “Stimulation Tests”

Sometimes, baseline blood tests aren’t enough. If your levels are “borderline,” your doctor may perform a stimulation test. Think of this as a “stress test” for your endocrine system [1].

  • The Insulin Tolerance Test (ITT): This is the “gold standard” [7]. You are given a small dose of insulin to lower your blood sugar. In a healthy body, this “stress” signals the pituitary to pump out growth hormone and cortisol. If your levels don’t rise, it confirms the pituitary is damaged [8]. Because lowering blood sugar deliberately sounds dangerous—especially since it can trigger crisis symptoms—this test is always conducted under strict, continuous medical supervision in a specialized setting to ensure your safety [7].
  • The ACTH Stimulation Test: A simpler, safer test where you are given a synthetic version of the hormone ACTH to see if your adrenal glands can still respond [9].

Imaging: The Pituitary MRI

While blood tests tell us how the gland is working, an MRI tells us what it looks like.

  • Acute Phase: If the scan is done shortly after birth, the pituitary may appear swollen or enlarged [10].
  • Chronic Phase: If the scan is done years later, the most common finding is an “empty sella” [11]. This means the pituitary gland has shrunken (atrophied) so much that the bony space where it sits (the sella turcica) looks empty on the scan [11][12].

Summary Checklist for Patients

Step What to Do Why It Matters
Records Gather delivery records and history of blood transfusions [3]. Proves the “triggering event” for the damage.
Lab Work Request a full “morning pituitary panel” [4]. Identifies which specific hormones are missing.
MRI Ask for a “Pituitary Protocol” MRI (not just a standard brain scan) [11]. Looks for the characteristic “empty sella.”

Common questions in this guide

How is Sheehan syndrome diagnosed?
Doctors diagnose Sheehan syndrome by reviewing your medical history for severe bleeding during childbirth, evaluating symptoms like a lack of breast milk, and performing specialized blood tests. A pituitary MRI is also often used to check for a shrunken pituitary gland.
What blood tests are needed to check for Sheehan syndrome?
Your doctor will typically order a morning hormone panel to check how your pituitary is functioning. This includes testing cortisol, thyroid hormones, estrogen, prolactin, and growth hormone to see which specific hormones are missing.
What does an empty sella mean on an MRI?
An empty sella is a common MRI finding in patients who have had Sheehan syndrome for years. It means the pituitary gland has shrunken so much that the bony space where it sits in the skull appears empty on the scan.
What is an Insulin Tolerance Test (ITT)?
An ITT is a stimulation test used to confirm pituitary damage. You are given a small dose of insulin to lower your blood sugar under strict medical supervision, which normally triggers your pituitary to release cortisol and growth hormone.
Do I need my past delivery records to get a diagnosis?
Yes, gathering your delivery records is highly recommended. Documenting a history of heavy blood loss or blood transfusions during childbirth helps prove the triggering event that caused the pituitary damage.

Questions to Ask Your Doctor

Curated prompts to bring to your next appointment.

  1. 1.Which specific hormone levels in my baseline blood work are the most concerning?
  2. 2.Is an Insulin Tolerance Test (ITT) necessary for my diagnosis, or can we use a safer alternative like the ACTH stimulation test?
  3. 3.Does my MRI show an 'empty sella,' and does the size of my pituitary gland match the severity of my symptoms?
  4. 4.Did you check my posterior pituitary function, such as my sodium and ADH levels?
  5. 5.Are there any specific 'cut-off' values you are using for my growth hormone and cortisol tests?

Questions For You

Tap a prompt to share your answer — we'll use it plus this page's context to start a tailored conversation.

References

References (12)
  1. 1

    Sheehan syndrome.

    Karaca Z, Laway BA, Dokmetas HS, et al.

    Nature reviews. Disease primers 2016; (2()):16092 doi:10.1038/nrdp.2016.92.

    PMID: 28004764
  2. 2

    A case of acute Sheehan's syndrome and literature review: a rare but life-threatening complication of postpartum hemorrhage.

    Matsuzaki S, Endo M, Ueda Y, et al.

    BMC pregnancy and childbirth 2017; (17(1)):188 doi:10.1186/s12884-017-1380-y.

    PMID: 28615049
  3. 3

    Sheehan's syndrome in Xinjiang: Clinical characteristics and laboratory evaluation of 97 patients.

    Du GL, Liu ZH, Chen M, et al.

    Hormones (Athens, Greece) 2015; (14(4)):660-7 doi:10.14310/horm.2002.1624.

    PMID: 26732159
  4. 4

    Delayed Sheehan's syndrome diagnosed during the evaluation of secondary infertility: A case report.

    Saxena S, Verma V, Samadarshi S, et al.

    Clinical case reports 2024; (12(2)):e8521 doi:10.1002/ccr3.8521.

    PMID: 38344342
  5. 5

    Adrenal crisis precipitated by influenza A led to the diagnosis of Sheehan's syndrome 18 years after postpartum hemorrhage.

    Taniguchi J, Sugawara H, Yamada H, et al.

    Clinical case reports 2020; (8(12)):3082-3087 doi:10.1002/ccr3.3355.

    PMID: 33363885
  6. 6

    Acute Sheehan Syndrome With Distinctive Arginine Vasopressin Secretion and Magnetic Resonance Imaging Findings.

    Minamoto Y, Sasai Y, Yamashita Y, et al.

    JCEM case reports 2025; (3(8)):luaf134 doi:10.1210/jcemcr/luaf134.

    PMID: 40657233
  7. 7

    The Cut-off Limits of Growth Hormone Response to the Insulin Tolerance Test Related to Body Mass Index for the Diagnosis of Adult Growth Hormone Deficiency.

    Gasco V, Ferrero A, Bisceglia A, et al.

    Neuroendocrinology 2021; (111(5)):442-450 doi:10.1159/000508103.

    PMID: 32335555
  8. 8

    Addison's disease concomitant with corticotropin deficiency and pituitary CRH resistance - a case report.

    Lewandowski KC, Malicka K, Dąbrowska K, Lewiński A

    Endokrynologia Polska 2017; (68(4)):468-471 doi:10.5603/EP.2017.0052.

    PMID: 28819949
  9. 9

    Total and free cortisol levels during 1 μg, 25 μg, and 250 μg cosyntropin stimulation tests compared to insulin tolerance test: results of a randomized, prospective, pilot study.

    Peechakara S, Bena J, Clarke NJ, et al.

    Endocrine 2017; (57(3)):388-393 doi:10.1007/s12020-017-1371-9.

    PMID: 28730418
  10. 10

    Pituitary haemorrhage and infarction: the spectrum of disease.

    Iqbal F, Adams W, Dimitropoulos I, et al.

    Endocrine connections 2021; (10(2)):171-179.

    PMID: 33434143
  11. 11

    A Rare Case of Sheehan Syndrome With Cardiac Tamponade.

    Zain A, Sivakumar A, Akah O, et al.

    Cureus 2022; (14(4)):e24329 doi:10.7759/cureus.24329.

    PMID: 35607582
  12. 12

    Acute pituitary disease in pregnancy: how to handle hypophysitis and Sheehan's syndrome.

    Honegger J, Giese S

    Minerva endocrinologica 2018; (43(4)):465-475 doi:10.23736/S0391-1977.18.02814-6.

    PMID: 29463076

This page explains the diagnostic process and lab testing for Sheehan syndrome for educational purposes only. Always consult your endocrinologist or healthcare provider for medical advice and test interpretation.

Get notified when new evidence is published on Sheehan syndrome.

We monitor PubMed for new peer-reviewed studies on this topic and email a short summary when something meaningful changes.